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nk cell isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec nk cell isolation kit
    Nk Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+nk+cell+isolation+kit/NK+Cell+Isolation+Kit%2C+mouse/pm42421015-59-8-12
    Average 96 stars, based on 391 article reviews
    nk cell isolation kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Nur77 agonism invigorates Natural Killer cell immunity against hepatocellular carcinoma.
    Article Snippet: Tissues were minced and enzymatically digested with Mouse Tumor Dissociation Kit (Miltenyi Biotec), then passed through a 70μm cell strainer to dissociate single cells from residual tissues. .. NK cells isolation was done using mouse NK cell isolation kit (Miltenyi Biotec) according to manufacturer’s protocol. .. Purified mouse NK cells were placed in AIM-V media (Gibco) supplemented with 5% Human Serum (Sigma), with an addition of 300 IU/mL recombinant IL-2 (Miltenyi Biotec).

    Article Title: Loss of EHMT2 enhances NK cell-driven anti-tumor immunity through TGF-β1 suppression
    Article Snippet: Spleens were dissected from female BALB/c mice, and a single-cell suspension was prepared using the Spleen Dissociation Kit (Miltenyi Biotec, Cat#130-095-926). .. NK cells were isolated using a Mouse NK Cell Isolation Kit (Miltenyi Biotec. ..

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. NK cells were isolated from C57BL/6J, Gzmb-/spleens and purified using a mouse NK cell isolation kit (130-115-818 Miltenyi Biotec; Paris, France) according to the manufacturer’s protocol. .. In brief, NK cells were negatively selected using a cocktail of antibody-coated magnetic beads followed by cell separation using LS magnetic columns (Miltenyi Biotec; Paris, France), yielding NK cells with >90% purity.

    Cell Isolation:

    Article Title: Nur77 agonism invigorates Natural Killer cell immunity against hepatocellular carcinoma.
    Article Snippet: Tissues were minced and enzymatically digested with Mouse Tumor Dissociation Kit (Miltenyi Biotec), then passed through a 70μm cell strainer to dissociate single cells from residual tissues. .. NK cells isolation was done using mouse NK cell isolation kit (Miltenyi Biotec) according to manufacturer’s protocol. .. Purified mouse NK cells were placed in AIM-V media (Gibco) supplemented with 5% Human Serum (Sigma), with an addition of 300 IU/mL recombinant IL-2 (Miltenyi Biotec).

    Article Title: Natural killer cells aggravate neuroinflammation through microglial type I interferon pathway activation after traumatic brain injury
    Article Snippet: .. NK cells were enriched using a magnetic associated cell sorting (MACS) method in combination with a negative selection protocol (Mouse NK cell isolation Kit II, cat no.130-096-892, Miltenyi Biotech GmbH, Germany) according to the manufacturer’s instructions. ..

    Article Title: Loss of EHMT2 enhances NK cell-driven anti-tumor immunity through TGF-β1 suppression
    Article Snippet: Spleens were dissected from female BALB/c mice, and a single-cell suspension was prepared using the Spleen Dissociation Kit (Miltenyi Biotec, Cat#130-095-926). .. NK cells were isolated using a Mouse NK Cell Isolation Kit (Miltenyi Biotec. ..

    Article Title: Synergistic immunotherapeutic effects of irreversible electroporation and CAR-NK cell therapy against hepatocellular carcinoma.
    Article Snippet: .. Following the manufacturer’s protocol, mouse primary NK cells (mNK) were extracted from mouse spleens using a commercially available mouse NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated mNK cells were then cultured in RPMI medium (HyClone) containing 10% FBS, 1% antibiotic/ antimycotic solution (Gibco), 2 mM L-glutamine, 1% nonessential amino acids (Gibco), and 50 μM 2-mercaptoethanol.

    Article Title: Synergistic immunotherapeutic effects of irreversible electroporation and CAR-NK cell therapy against hepatocellular carcinoma
    Article Snippet: .. Following the manufacturer’s protocol, mouse primary NK cells (mNK) were extracted from mouse spleens using a commercially available mouse NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated mNK cells were then cultured in RPMI medium (HyClone) containing 10% FBS, 1% antibiotic/antimycotic solution (Gibco), 2 mM L-glutamine, 1% nonessential amino acids (Gibco), and 50 μM 2-mercaptoethanol.

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. NK cells were isolated from C57BL/6J, Gzmb-/spleens and purified using a mouse NK cell isolation kit (130-115-818 Miltenyi Biotec; Paris, France) according to the manufacturer’s protocol. .. In brief, NK cells were negatively selected using a cocktail of antibody-coated magnetic beads followed by cell separation using LS magnetic columns (Miltenyi Biotec; Paris, France), yielding NK cells with >90% purity.

    FACS:

    Article Title: Natural killer cells aggravate neuroinflammation through microglial type I interferon pathway activation after traumatic brain injury
    Article Snippet: .. NK cells were enriched using a magnetic associated cell sorting (MACS) method in combination with a negative selection protocol (Mouse NK cell isolation Kit II, cat no.130-096-892, Miltenyi Biotech GmbH, Germany) according to the manufacturer’s instructions. ..

    Magnetic Cell Separation:

    Article Title: Natural killer cells aggravate neuroinflammation through microglial type I interferon pathway activation after traumatic brain injury
    Article Snippet: .. NK cells were enriched using a magnetic associated cell sorting (MACS) method in combination with a negative selection protocol (Mouse NK cell isolation Kit II, cat no.130-096-892, Miltenyi Biotech GmbH, Germany) according to the manufacturer’s instructions. ..

    Selection:

    Article Title: Natural killer cells aggravate neuroinflammation through microglial type I interferon pathway activation after traumatic brain injury
    Article Snippet: .. NK cells were enriched using a magnetic associated cell sorting (MACS) method in combination with a negative selection protocol (Mouse NK cell isolation Kit II, cat no.130-096-892, Miltenyi Biotech GmbH, Germany) according to the manufacturer’s instructions. ..

    Purification:

    Article Title: NK cells promote cardiac cell death and regulate myelopoiesis in myocardial infarction.
    Article Snippet: .. NK cells were isolated from C57BL/6J, Gzmb-/spleens and purified using a mouse NK cell isolation kit (130-115-818 Miltenyi Biotec; Paris, France) according to the manufacturer’s protocol. .. In brief, NK cells were negatively selected using a cocktail of antibody-coated magnetic beads followed by cell separation using LS magnetic columns (Miltenyi Biotec; Paris, France), yielding NK cells with >90% purity.

    other:

    Article Title: Cross-species cellular mapping and humanization of Fcγ receptors to advance antibody modeling.
    Article Snippet: The cell line was cultured in RPMI 1640 medium supplemented with 10% heat- inactivated fetal bovine serum, 2 mM l- glutamine, 1% nonessential amino acids, and 1 mM sodium pyruvate (all from Gibco) and maintained at 37°C under an atmosphere containing 5% CO2.

    Article Title: IL-10 secretion by CD8 T cells orchestrates early NK cell recruitment and antitumor immunity after anti-VEGFR-2/PD-1 therapy.
    Article Snippet: Bioluminescence images were analyzed using Living Image software (Perkin Elmer), and results were represented as fold- change in bioluminescence.



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